Draft
International Standard
ISO/DIS 13136-2
Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) — Part 2: Horizontal method for the characterization of Shiga toxin-producing Escherichia coli (STEC) isolates
Reference number
ISO/DIS 13136-2
Edition 1
Proyecto Norma internacional
Preview
ISO/DIS 13136-2
84814
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Este borrador de Norma Internacional se encuentra en la fase de consultas con los miembros de ISO.
Reemplazará ISO/TS 13136:2012

ISO/DIS 13136-2

ISO/DIS 13136-2
84814
Idioma
Formato
CHF 63
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Resumen

This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard: - subtyping of the Stx-coding genes; - determination of the presence of eae gene; - determination of the presence of aggR gene; - identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45. The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.

Informaciones generales

  •  : En desarrollo
    : Cierre de la votación [40.60]
  •  : 1
     : 19
  • ISO/TC 34/SC 9
    07.100.30 
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